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hela cell line  (ATCC)


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    ATCC hela cell line
    Mammalian cell-based characterization of HiCaRI ( A ) Image of <t>HeLa</t> cells expressing HiCaRI in the cytosol from a Ca 2+ -depleted (4 mM EGTA, 1.5 µM ionomycin, shown left) to a Ca 2+ saturated state (10 mM CaCl 2 , 1.5 µM ionomycin, shown right). Scale bar = 20 µm. ( B ) Three-step Ca 2+ fluorescence response of HiCaRI (5 µM histamine stimulation followed by 4 mM EGTA, 1.5 µM ionomycin, then 10 mM CaCl 2 , 1.5 µM ionomycin. Cells were seeded prior to EGTA and CaCl 2 addition. ( C ) Boxplot of Δ F / F min calculated from Ca 2+ depletion to Ca 2+ saturation over all sampled HeLa cells expressing HiCaRI in the cytosol ( n = 74, n = 3 biological replicates, mean ± s.e.m). ( D ) Photostability comparison of H2B-HiCaRI and H2B-jGCaMP8s in the presence and absence of Ca 2+ . HeLa cells transfected with H2B-jGCaMP8s or H2B-HiCaRI were continuously illuminated (4.8 mW cm −2 at 470 ± 20 nm) for 500 or 1200 seconds. ( n = 3 biological replicates, mean ± s.e.m.).
    Hela Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A StayGold-based calcium ion indicator"

    Article Title: A StayGold-based calcium ion indicator

    Journal: bioRxiv

    doi: 10.64898/2026.03.06.710044

    Mammalian cell-based characterization of HiCaRI ( A ) Image of HeLa cells expressing HiCaRI in the cytosol from a Ca 2+ -depleted (4 mM EGTA, 1.5 µM ionomycin, shown left) to a Ca 2+ saturated state (10 mM CaCl 2 , 1.5 µM ionomycin, shown right). Scale bar = 20 µm. ( B ) Three-step Ca 2+ fluorescence response of HiCaRI (5 µM histamine stimulation followed by 4 mM EGTA, 1.5 µM ionomycin, then 10 mM CaCl 2 , 1.5 µM ionomycin. Cells were seeded prior to EGTA and CaCl 2 addition. ( C ) Boxplot of Δ F / F min calculated from Ca 2+ depletion to Ca 2+ saturation over all sampled HeLa cells expressing HiCaRI in the cytosol ( n = 74, n = 3 biological replicates, mean ± s.e.m). ( D ) Photostability comparison of H2B-HiCaRI and H2B-jGCaMP8s in the presence and absence of Ca 2+ . HeLa cells transfected with H2B-jGCaMP8s or H2B-HiCaRI were continuously illuminated (4.8 mW cm −2 at 470 ± 20 nm) for 500 or 1200 seconds. ( n = 3 biological replicates, mean ± s.e.m.).
    Figure Legend Snippet: Mammalian cell-based characterization of HiCaRI ( A ) Image of HeLa cells expressing HiCaRI in the cytosol from a Ca 2+ -depleted (4 mM EGTA, 1.5 µM ionomycin, shown left) to a Ca 2+ saturated state (10 mM CaCl 2 , 1.5 µM ionomycin, shown right). Scale bar = 20 µm. ( B ) Three-step Ca 2+ fluorescence response of HiCaRI (5 µM histamine stimulation followed by 4 mM EGTA, 1.5 µM ionomycin, then 10 mM CaCl 2 , 1.5 µM ionomycin. Cells were seeded prior to EGTA and CaCl 2 addition. ( C ) Boxplot of Δ F / F min calculated from Ca 2+ depletion to Ca 2+ saturation over all sampled HeLa cells expressing HiCaRI in the cytosol ( n = 74, n = 3 biological replicates, mean ± s.e.m). ( D ) Photostability comparison of H2B-HiCaRI and H2B-jGCaMP8s in the presence and absence of Ca 2+ . HeLa cells transfected with H2B-jGCaMP8s or H2B-HiCaRI were continuously illuminated (4.8 mW cm −2 at 470 ± 20 nm) for 500 or 1200 seconds. ( n = 3 biological replicates, mean ± s.e.m.).

    Techniques Used: Cell Characterization, Expressing, Fluorescence, Comparison, Transfection



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    Mammalian cell-based characterization of HiCaRI ( A ) Image of <t>HeLa</t> cells expressing HiCaRI in the cytosol from a Ca 2+ -depleted (4 mM EGTA, 1.5 µM ionomycin, shown left) to a Ca 2+ saturated state (10 mM CaCl 2 , 1.5 µM ionomycin, shown right). Scale bar = 20 µm. ( B ) Three-step Ca 2+ fluorescence response of HiCaRI (5 µM histamine stimulation followed by 4 mM EGTA, 1.5 µM ionomycin, then 10 mM CaCl 2 , 1.5 µM ionomycin. Cells were seeded prior to EGTA and CaCl 2 addition. ( C ) Boxplot of Δ F / F min calculated from Ca 2+ depletion to Ca 2+ saturation over all sampled HeLa cells expressing HiCaRI in the cytosol ( n = 74, n = 3 biological replicates, mean ± s.e.m). ( D ) Photostability comparison of H2B-HiCaRI and H2B-jGCaMP8s in the presence and absence of Ca 2+ . HeLa cells transfected with H2B-jGCaMP8s or H2B-HiCaRI were continuously illuminated (4.8 mW cm −2 at 470 ± 20 nm) for 500 or 1200 seconds. ( n = 3 biological replicates, mean ± s.e.m.).
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    Mammalian cell-based characterization of HiCaRI ( A ) Image of HeLa cells expressing HiCaRI in the cytosol from a Ca 2+ -depleted (4 mM EGTA, 1.5 µM ionomycin, shown left) to a Ca 2+ saturated state (10 mM CaCl 2 , 1.5 µM ionomycin, shown right). Scale bar = 20 µm. ( B ) Three-step Ca 2+ fluorescence response of HiCaRI (5 µM histamine stimulation followed by 4 mM EGTA, 1.5 µM ionomycin, then 10 mM CaCl 2 , 1.5 µM ionomycin. Cells were seeded prior to EGTA and CaCl 2 addition. ( C ) Boxplot of Δ F / F min calculated from Ca 2+ depletion to Ca 2+ saturation over all sampled HeLa cells expressing HiCaRI in the cytosol ( n = 74, n = 3 biological replicates, mean ± s.e.m). ( D ) Photostability comparison of H2B-HiCaRI and H2B-jGCaMP8s in the presence and absence of Ca 2+ . HeLa cells transfected with H2B-jGCaMP8s or H2B-HiCaRI were continuously illuminated (4.8 mW cm −2 at 470 ± 20 nm) for 500 or 1200 seconds. ( n = 3 biological replicates, mean ± s.e.m.).

    Journal: bioRxiv

    Article Title: A StayGold-based calcium ion indicator

    doi: 10.64898/2026.03.06.710044

    Figure Lengend Snippet: Mammalian cell-based characterization of HiCaRI ( A ) Image of HeLa cells expressing HiCaRI in the cytosol from a Ca 2+ -depleted (4 mM EGTA, 1.5 µM ionomycin, shown left) to a Ca 2+ saturated state (10 mM CaCl 2 , 1.5 µM ionomycin, shown right). Scale bar = 20 µm. ( B ) Three-step Ca 2+ fluorescence response of HiCaRI (5 µM histamine stimulation followed by 4 mM EGTA, 1.5 µM ionomycin, then 10 mM CaCl 2 , 1.5 µM ionomycin. Cells were seeded prior to EGTA and CaCl 2 addition. ( C ) Boxplot of Δ F / F min calculated from Ca 2+ depletion to Ca 2+ saturation over all sampled HeLa cells expressing HiCaRI in the cytosol ( n = 74, n = 3 biological replicates, mean ± s.e.m). ( D ) Photostability comparison of H2B-HiCaRI and H2B-jGCaMP8s in the presence and absence of Ca 2+ . HeLa cells transfected with H2B-jGCaMP8s or H2B-HiCaRI were continuously illuminated (4.8 mW cm −2 at 470 ± 20 nm) for 500 or 1200 seconds. ( n = 3 biological replicates, mean ± s.e.m.).

    Article Snippet: The HeLa cell line was purchased from ATCC (#CCL-2).

    Techniques: Cell Characterization, Expressing, Fluorescence, Comparison, Transfection